|
KMID : 0043320080310050628
|
|
Archives of Pharmacal Research
2008 Volume.31 No. 5 p.628 ~ p.633
|
|
|
Dioleoyl Phosphatidic Acid Induces Morphological Changes through an Endogenous LPA Receptor in C6 Glioma Cells
|
|
Chang Young-Ja
Kim Yu-Lee
Jo Ji-Yeong
Kim Kye-Ok
Kim Hyo-Lim
Im Dong-Soon
|
|
|
Abstract
|
|
|
|
Previously, we suggested that dioleoyl phosphatidic acid (PA) and lysophosphatidic acid (LPA) increased through endogenous LPA receptors coupled to pertussis toxin-sensitive G proteins in rat C6 glioma cells. In the present report, we investigated morphological changes and cytotoxicity induced by PA and LPA in C6 glioma cells. Isoproterenol treatment led to changes in the cell morphology of rat C6 glioma cells, which were reverted by the addition of PA and LPA. PA- and LPA-induced morphological reversions were inhibited by treatment with Ki16425, an receptor antagonist. VPC32183, another receptor antagonist with a different structure, only inhibited PA-induced morphological reversion but not LPAinduced reversion. However, the reversions were not inhibited by treatment with pertussis toxin, a specific inhibitor of proteins. In addition, cytotoxicity was only induced by LPA but not by PA in C6 glioma cells. Our results suggest that PA may act as a partial agonist at endogenous LPA receptors, which are sensitive to Ki16425 and coupled to PTX-insensitive G proteins, to evoke morphological changes in C6 glioma cells.
|
|
|
KEYWORD
|
|
|
Phosphatidic acid, Lysophosphatidic acid, G-Protein-coupled receptor
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|